Our Technology

Single Sperm Intracytoplasmic Injection (also known as Intracytoplasmic Sperm Injection, or ICSI)  is a micromanipulation technique in the field of assisted reproduction that involves directly injecting a single sperm into the cytoplasm of an oocyte using a microscopic system to achieve fertilization. 

The technical principle and procedure include selecting a single sperm and microscopically injecting it into a mouse (or other mammalian) oocyte, culturing it in vitro until the early embryo stage, and then transferring the embryo back into the maternal uterus for implantation and development. Clinically and in research, it is mainly used to increase fertilization rates and to address difficulties in conception caused by insufficient sperm motility or poor sperm quality in traditional in vitro fertilization (IVF). 

The main advantages of ICSI technology include:

•  Simplified fertilization process: Each oocyte receives injection of one sperm, avoiding polyspermy, bypassing complex natural fertilization steps such as sperm-egg binding and capacitation, effectively shortening fertilization time. 

•  Reduced requirement for sperm motility: Fertilization can be achieved even with sperm that have very low motility or are immotile, as long as the genetic material is intact. This makes it suitable for infertility caused by sperm abnormalities or male reproductive disorders. 

• Application in difficult-to-breed strains: For example, male-sterile genetically      modified strains or strains with poor sperm motility after cryopreservation recovery; ICSI can successfully produce offspring and rescue breeding difficulties.

•  Versatility for diverse research needs: Other exogenous substances can be co-injected during ICSI, meeting complex research demands and promoting experimental animal model and gene function studies. 

In summary, ICSI is a key technique in experimental animal models and assisted reproduction, especially suited for overcoming fertilization difficulties due to sperm quality or motility issues. It is an important tool that advances reproductive biology and gene editing research.